Review



human foreskin fibroblast bj  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC human foreskin fibroblast bj
    (A) BF images five out of the seven cell lines, including telomerase-negative <t>fibroblasts</t> (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.
    Human Foreskin Fibroblast Bj, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1702 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblast bj/product/ATCC
    Average 99 stars, based on 1702 article reviews
    human foreskin fibroblast bj - by Bioz Stars, 2026-06
    99/100 stars

    Images

    1) Product Images from "Epigenetic–splicing regulation of hTERT mediated by hTAPAS"

    Article Title: Epigenetic–splicing regulation of hTERT mediated by hTAPAS

    Journal: bioRxiv

    doi: 10.64898/2026.05.08.723733

    (A) BF images five out of the seven cell lines, including telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.
    Figure Legend Snippet: (A) BF images five out of the seven cell lines, including telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.

    Techniques Used: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Expressing

    Targeted DNA methylation profiling was performed using CRISPR–Cas9 enrichment followed by Nanopore sequencing across a ∼9 kb region spanning hTAPAS through hTERT intron 2 (Chr. 5: 1,196,006–1,205,206) and a ∼6.5 kb region covering introns 6–8 (Chr. 5: 1,174,035–1,180,535). The analysis included telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive cell lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive cell line VA13. DNA methylation levels at individual CpG sites are depicted across the indicated genomic regions, including hTAPAS , the THOR region, the core promoter, exon1, intron1, exon 2 and intron 2. Methylation for each individual CpG is shown as a percentage, with unmethylated CpGs depicted in red and methylated CpGs in blue. Methylation across intron 2 was consistently high (80–100%) in all cell lines, whereas regions encompassing hTAPAS , the THOR region, exon 2, and introns 6–8 displayed marked variability between cell types. CpGs within the hTAPAS region were highly methylated in telomerase-positive cells and in the ALT-positive VA13 line, but largely unmethylated in fibroblasts, with partial methylation observed in IMR90. The core hTERT promoter and exon 2–proximal regions remained mostly unmethylated in all cell lines except VA13, which exhibited substantial hypermethylation
    Figure Legend Snippet: Targeted DNA methylation profiling was performed using CRISPR–Cas9 enrichment followed by Nanopore sequencing across a ∼9 kb region spanning hTAPAS through hTERT intron 2 (Chr. 5: 1,196,006–1,205,206) and a ∼6.5 kb region covering introns 6–8 (Chr. 5: 1,174,035–1,180,535). The analysis included telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive cell lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive cell line VA13. DNA methylation levels at individual CpG sites are depicted across the indicated genomic regions, including hTAPAS , the THOR region, the core promoter, exon1, intron1, exon 2 and intron 2. Methylation for each individual CpG is shown as a percentage, with unmethylated CpGs depicted in red and methylated CpGs in blue. Methylation across intron 2 was consistently high (80–100%) in all cell lines, whereas regions encompassing hTAPAS , the THOR region, exon 2, and introns 6–8 displayed marked variability between cell types. CpGs within the hTAPAS region were highly methylated in telomerase-positive cells and in the ALT-positive VA13 line, but largely unmethylated in fibroblasts, with partial methylation observed in IMR90. The core hTERT promoter and exon 2–proximal regions remained mostly unmethylated in all cell lines except VA13, which exhibited substantial hypermethylation

    Techniques Used: DNA Methylation Assay, CRISPR, Nanopore Sequencing, Methylation



    Similar Products

    human foreskin fibroblast bj  (ATCC)
    99
    ATCC human foreskin fibroblast bj
    (A) BF images five out of the seven cell lines, including telomerase-negative <t>fibroblasts</t> (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.
    Human Foreskin Fibroblast Bj, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblast bj/product/ATCC
    Average 99 stars, based on 1 article reviews
    human foreskin fibroblast bj - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    human foreskin fibroblast bj cells  (ATCC)
    99
    ATCC human foreskin fibroblast bj cells
    (A) BF images five out of the seven cell lines, including telomerase-negative <t>fibroblasts</t> (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.
    Human Foreskin Fibroblast Bj Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblast bj cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    human foreskin fibroblast bj cells - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    human foreskin fibroblast cells  (ATCC)
    96
    ATCC human foreskin fibroblast cells
    (A) BF images five out of the seven cell lines, including telomerase-negative <t>fibroblasts</t> (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.
    Human Foreskin Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblast cells/product/ATCC
    Average 96 stars, based on 1 article reviews
    human foreskin fibroblast cells - by Bioz Stars, 2026-06
    96/100 stars
    		  Buy from Supplier
    human bj fibroblasts  (ATCC)
    99
    ATCC human bj fibroblasts
    (A) BF images five out of the seven cell lines, including telomerase-negative <t>fibroblasts</t> (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.
    Human Bj Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human bj fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    human bj fibroblasts - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    human fibroblast cells  (ATCC)
    96
    ATCC human fibroblast cells
    (A) BF images five out of the seven cell lines, including telomerase-negative <t>fibroblasts</t> (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.
    Human Fibroblast Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human fibroblast cells/product/ATCC
    Average 96 stars, based on 1 article reviews
    human fibroblast cells - by Bioz Stars, 2026-06
    96/100 stars
    		  Buy from Supplier
    normal human fibroblasts  (ATCC)
    99
    ATCC normal human fibroblasts
    (A) BF images five out of the seven cell lines, including telomerase-negative <t>fibroblasts</t> (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.
    Normal Human Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/normal human fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    normal human fibroblasts - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    human dermal fibroblasts  (ATCC)
    99
    ATCC human dermal fibroblasts
    (A) BF images five out of the seven cell lines, including telomerase-negative <t>fibroblasts</t> (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.
    Human Dermal Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human dermal fibroblasts/product/ATCC
    Average 99 stars, based on 1 article reviews
    human dermal fibroblasts - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier
    fibroblast human normal cell line bj 1  (ATCC)
    99
    ATCC fibroblast human normal cell line bj 1
    (A) BF images five out of the seven cell lines, including telomerase-negative <t>fibroblasts</t> (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.
    Fibroblast Human Normal Cell Line Bj 1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibroblast human normal cell line bj 1/product/ATCC
    Average 99 stars, based on 1 article reviews
    fibroblast human normal cell line bj 1 - by Bioz Stars, 2026-06
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) BF images five out of the seven cell lines, including telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.

    Journal: bioRxiv

    Article Title: Epigenetic–splicing regulation of hTERT mediated by hTAPAS

    doi: 10.64898/2026.05.08.723733

    Figure Lengend Snippet: (A) BF images five out of the seven cell lines, including telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive line VA13. (B) Quantification of hTAPAS and hTERT mRNA levels by qRT-PCR. (C) RT–PCR analysis of hTERT splice isoforms using primers spanning exons 2–3 and 5–9. An inverse relationship between hTAPAS and hTERT expression was observed in HFF-1 and BJ fibroblasts (low hTERT , detectable hTAPAS ) and in HEK293T and NALM6 cells (high hTERT , absent hTAPAS ), whereas intermediate patterns were detected in IMR90, VA13, and iPSCs, with iPSCs maintaining moderate hTERT expression despite high hTAPAS levels.

    Article Snippet: Human embryonic kidney 293T (HEK293T; ATCC® CRL-3216TM), human embryonic lung fibroblast VA13 (WI-38 VA13 subline 2RA; ATCC® CCL-75.1TM), human foreskin fibroblast HFF-1 (ATCC® SCRC-1041TM), human lung fibroblast IMR-90 (ATCC® CCL-186TM), and human foreskin fibroblast BJ (ATCC® CRL-2522TM) cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction, Expressing

    Targeted DNA methylation profiling was performed using CRISPR–Cas9 enrichment followed by Nanopore sequencing across a ∼9 kb region spanning hTAPAS through hTERT intron 2 (Chr. 5: 1,196,006–1,205,206) and a ∼6.5 kb region covering introns 6–8 (Chr. 5: 1,174,035–1,180,535). The analysis included telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive cell lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive cell line VA13. DNA methylation levels at individual CpG sites are depicted across the indicated genomic regions, including hTAPAS , the THOR region, the core promoter, exon1, intron1, exon 2 and intron 2. Methylation for each individual CpG is shown as a percentage, with unmethylated CpGs depicted in red and methylated CpGs in blue. Methylation across intron 2 was consistently high (80–100%) in all cell lines, whereas regions encompassing hTAPAS , the THOR region, exon 2, and introns 6–8 displayed marked variability between cell types. CpGs within the hTAPAS region were highly methylated in telomerase-positive cells and in the ALT-positive VA13 line, but largely unmethylated in fibroblasts, with partial methylation observed in IMR90. The core hTERT promoter and exon 2–proximal regions remained mostly unmethylated in all cell lines except VA13, which exhibited substantial hypermethylation

    Journal: bioRxiv

    Article Title: Epigenetic–splicing regulation of hTERT mediated by hTAPAS

    doi: 10.64898/2026.05.08.723733

    Figure Lengend Snippet: Targeted DNA methylation profiling was performed using CRISPR–Cas9 enrichment followed by Nanopore sequencing across a ∼9 kb region spanning hTAPAS through hTERT intron 2 (Chr. 5: 1,196,006–1,205,206) and a ∼6.5 kb region covering introns 6–8 (Chr. 5: 1,174,035–1,180,535). The analysis included telomerase-negative fibroblasts (HFF-1, IMR90, BJ), telomerase-positive cell lines (HEK293T, NALM6, HG002 iPSCs), and the ALT-positive cell line VA13. DNA methylation levels at individual CpG sites are depicted across the indicated genomic regions, including hTAPAS , the THOR region, the core promoter, exon1, intron1, exon 2 and intron 2. Methylation for each individual CpG is shown as a percentage, with unmethylated CpGs depicted in red and methylated CpGs in blue. Methylation across intron 2 was consistently high (80–100%) in all cell lines, whereas regions encompassing hTAPAS , the THOR region, exon 2, and introns 6–8 displayed marked variability between cell types. CpGs within the hTAPAS region were highly methylated in telomerase-positive cells and in the ALT-positive VA13 line, but largely unmethylated in fibroblasts, with partial methylation observed in IMR90. The core hTERT promoter and exon 2–proximal regions remained mostly unmethylated in all cell lines except VA13, which exhibited substantial hypermethylation

    Article Snippet: Human embryonic kidney 293T (HEK293T; ATCC® CRL-3216TM), human embryonic lung fibroblast VA13 (WI-38 VA13 subline 2RA; ATCC® CCL-75.1TM), human foreskin fibroblast HFF-1 (ATCC® SCRC-1041TM), human lung fibroblast IMR-90 (ATCC® CCL-186TM), and human foreskin fibroblast BJ (ATCC® CRL-2522TM) cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: DNA Methylation Assay, CRISPR, Nanopore Sequencing, Methylation